产品货号：
Z38826
中文名称：
HSP60/HSPD1抗体
英文名称：
Anti-HSP60/HSPD1 Antibody (Clone#6G2)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-HSP60/HSPD1 Antibody (Clone#6G2) |
| 指标别名 | HSP 60;HSP60;HSP65;HSPD1 |
| 克隆性 | Monoclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,FCM |
| 基因名称 | HSPD1 |
| 克隆号 | 6G2 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG1 |
| 免疫原 | E.coli-derived human Hsp60/HSPD1 recombinant protein (Position:A260-Q496).Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60. |
| 实际分子量 | 60KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | HSP60 is a member of the chaperonin class of protein factors,which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts.It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells,which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells.HSP60 enhances Treg activity via TLR2,leading to activation of an intracellular signaling cascade that included p38,as well as inhibition of ERK phosphorylation.Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10,and it leads to downregulation of ERK,NFKB,and TBET expression.The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling. |
| Uniprot ID | P10809 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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